생물학 논문 해석, hierarchy의 의미가 뭔가요?
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논문에서 피규어 해석이 안되서 질문드립니다.
대충 저 피규어 설명을 보면,
We previously identified a stem proteomic f‐LSC profile (SPP) by two‐dimensional gel electrophoresis (2D‐IPG) including some wound healing proteins, that is profilin‐1, cofilin, vinculin and grp78, lectin‐1 and thioredoxin‐1 (txn1) (Supporting Information 2, Figure 1). To elu‐ cidate the association among the stem transcriptional triad and the stem proteomic f‐LSC profile, several gene‐silencing experiments were performed. The f‐LSCs were incubated with oct‐4, sox2 and nanog siRNA individually up to 72 hours, and finally the mRNA lev‐ els of gene coding for the SPP were evaluated (Figure 1A). Relative expression analysis revealed a significant modification in the fold change (FC) values for all stem gene markers (Supporting Information 2, Table 4). In particular, in siRNA‐transfected f‐LSCs significant re‐ duction in the mRNA levels of profilin‐1 and cofilin‐1 was detected, together with an appropriate elevation of vinculin mRNA levels, when compared to the control f‐LSCs. In detail, profilin‐1 mRNA lev‐ els were decreased up to 0.49 ± 0.09, 0.29 ± 0.07 and 0.06 ± 0.012 FC in oct4, sox2 and nanog siRNA‐transfected f‐LSCs, respectively (P < 0.001) when compared to the no‐target siRNA f‐LSCs.
To understand if a hierarchy exists behind the regulation of the stem transcriptional triad, we observed the behaviour of all three genes after silencing each of them up to 72 hours. The efficiency for gene silencing experiments of oct4, sox2, nanog was 91.51%, 88,72% and 83.32% respectively (represented in Figure 1B,C by # sign). I
여기서
1. *** To understand if a hierarchy exists behind the regulation of the stem transcriptional triad, 이부분이 이해가 잘 가지 않습니다. ㅠㅠ 왜 hierarchy(계층) 이 존재하는지 알아본거죠? 혹시 A번 피규어에서 profilin-1과 confilin은 감소하고 vinculin은 증가했는데 그것과 관련이 있는것인가요? 어떻게 연관시켜서 이해해야 할지 모르겠습니다. ㅠ 저것들이 증가 감소한것과 hirearchy의 존재를 확인하는게 어떤 의미가 있는건가요? 그 맥락을 좀 설명좀 해주실 수 있을까요?
또
2. *** f‐LSC profile 라는 부분에서 profile을 어떻게 해석해야 할까요? 의미를 어떻게 받아들여야 할지 알려주세요 ㅠㅠ
논문에서 피규어 해석이 안되서 질문드립니다.
대충 저 피규어 설명을 보면,
We previously identified a stem proteomic f‐LSC profile (SPP) by two‐dimensional gel electrophoresis (2D‐IPG) including some wound healing proteins, that is profilin‐1, cofilin, vinculin and grp78, lectin‐1 and thioredoxin‐1 (txn1) (Supporting Information 2, Figure 1). To elu‐ cidate the association among the stem transcriptional triad and the stem proteomic f‐LSC profile, several gene‐silencing experiments were performed. The f‐LSCs were incubated with oct‐4, sox2 and nanog siRNA individually up to 72 hours, and finally the mRNA lev‐ els of gene coding for the SPP were evaluated (Figure 1A). Relative expression analysis revealed a significant modification in the fold change (FC) values for all stem gene markers (Supporting Information 2, Table 4). In particular, in siRNA‐transfected f‐LSCs significant re‐ duction in the mRNA levels of profilin‐1 and cofilin‐1 was detected, together with an appropriate elevation of vinculin mRNA levels, when compared to the control f‐LSCs. In detail, profilin‐1 mRNA lev‐ els were decreased up to 0.49 ± 0.09, 0.29 ± 0.07 and 0.06 ± 0.012 FC in oct4, sox2 and nanog siRNA‐transfected f‐LSCs, respectively (P < 0.001) when compared to the no‐target siRNA f‐LSCs.
To understand if a hierarchy exists behind the regulation of the stem transcriptional triad, we observed the behaviour of all three genes after silencing each of them up to 72 hours. The efficiency for gene silencing experiments of oct4, sox2, nanog was 91.51%, 88,72% and 83.32% respectively (represented in Figure 1B,C by # sign). I
여기서
1. *** To understand if a hierarchy exists behind the regulation of the stem transcriptional triad, 이부분이 이해가 잘 가지 않습니다. ㅠㅠ 왜 hierarchy(계층) 이 존재하는지 알아본거죠? 혹시 A번 피규어에서 profilin-1과 confilin은 감소하고 vinculin은 증가했는데 그것과 관련이 있는것인가요? 어떻게 연관시켜서 이해해야 할지 모르겠습니다. ㅠ 저것들이 증가 감소한것과 hirearchy의 존재를 확인하는게 어떤 의미가 있는건가요? 그 맥락을 좀 설명좀 해주실 수 있을까요?
또
2. *** f‐LSC profile 라는 부분에서 profile을 어떻게 해석해야 할까요? 의미를 어떻게 받아들여야 할지 알려주세요 ㅠㅠ
#생물학 논문 #생물학 논문 사이트 #생물학 논문 읽기 #생물학 관련 논문 #생물학 박사 논문 #생물학 석사 논문 #합성 생물학 논문 #일반 생물학 논문
