미생물관련 번역...내공걸께요^^
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게시물 수정 , 삭제는 로그인 필요
세미나 발표를 준비중인데 번역이 잘 안되네요...^^;;
내용이 너무 많아 잘라서 올렸습니다.
너무 길면 싫어하실까봐...ㅜ.ㅠ
꼭 부탁드립니다.
2004년 논문으로 제목은...
Application of Flow Cytometry to Monitoring of Liposomal Restructuring Induced by Listeria monocytogenes
입니다.
Listeria monocytogenes NCTC 7973 hemolytic and non-hemolytic derivatives (13) and Escherichia coli NCIB 10772 (non-hemolytic) were obtained from the Department of Life Sciences, King's College London. The hemolytic activity of each strain was checked by subculturing the bacteria on horse blood agar plate (Unipath). Approximately 50 ml of sterile BHI broth (Oxoid) was inoculated with 0.5 ml of an overnight grown bacterial culture and incubated aerobically in a shaking incubator (200rev min-1) at 37℃. Early stationary phase bacteria were harvested by centrifugation (10,000×ｇ, 15min, 4℃) and washed three times in a buffer (0.01 M sodium phosphate buffer, pH 7.0). The washed bacteria were resuspended in the buffer to the desired concentration of bacteria, estimated from a preestablished bacterial concentration of bacteria, estimated from a preestablished bacteria concentration calibration curve (colony forming unit, cfu ml-1 vs. O.D. at 600nm) for each strain.
Fluorescent dye-entrapped small unilamella vesicles (SUVs) were prepared with commercially available pure phosphatidylcholine (PC), from egg yolk, phosphatidylcholine content 99%: Sigma) and calcein (Sigma). The PC solution was dried in a vacuum evaporator for 1h at 60rpm at room temperature. After all the solvent was removed, the PC film was hydrated, using 0.08 M calcein-buffer (0.01 M Na-phosphate, pH 7.0) sulution. SUVs were prepared by sonication of PC-calcein suspension with a sonicator (Branson, Model 250; power setting: 5; duty cycle: 30%, 12min). The emulsion was then passed through a Sephadex (G-50, Sigma) column to purify fractions containing calcein-entrapped liposomes (7, 15).
세미나 발표를 준비중인데 번역이 잘 안되네요...^^;;
내용이 너무 많아 잘라서 올렸습니다.
너무 길면 싫어하실까봐...ㅜ.ㅠ
꼭 부탁드립니다.
2004년 논문으로 제목은...
Application of Flow Cytometry to Monitoring of Liposomal Restructuring Induced by Listeria monocytogenes
입니다.
Listeria monocytogenes NCTC 7973 hemolytic and non-hemolytic derivatives (13) and Escherichia coli NCIB 10772 (non-hemolytic) were obtained from the Department of Life Sciences, King's College London. The hemolytic activity of each strain was checked by subculturing the bacteria on horse blood agar plate (Unipath). Approximately 50 ml of sterile BHI broth (Oxoid) was inoculated with 0.5 ml of an overnight grown bacterial culture and incubated aerobically in a shaking incubator (200rev min-1) at 37℃. Early stationary phase bacteria were harvested by centrifugation (10,000×ｇ, 15min, 4℃) and washed three times in a buffer (0.01 M sodium phosphate buffer, pH 7.0). The washed bacteria were resuspended in the buffer to the desired concentration of bacteria, estimated from a preestablished bacterial concentration of bacteria, estimated from a preestablished bacteria concentration calibration curve (colony forming unit, cfu ml-1 vs. O.D. at 600nm) for each strain.
Fluorescent dye-entrapped small unilamella vesicles (SUVs) were prepared with commercially available pure phosphatidylcholine (PC), from egg yolk, phosphatidylcholine content 99%: Sigma) and calcein (Sigma). The PC solution was dried in a vacuum evaporator for 1h at 60rpm at room temperature. After all the solvent was removed, the PC film was hydrated, using 0.08 M calcein-buffer (0.01 M Na-phosphate, pH 7.0) sulution. SUVs were prepared by sonication of PC-calcein suspension with a sonicator (Branson, Model 250; power setting: 5; duty cycle: 30%, 12min). The emulsion was then passed through a Sephadex (G-50, Sigma) column to purify fractions containing calcein-entrapped liposomes (7, 15).
