plaque assay 플라크 잡종화 방법

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고등학생입니다.
박테리오파지가 대장균을 얼마나 죽일 수 있는지 관찰하는 실험을 하려는데, 디스크 확산법이나 염색하는 것보다 plaque assay가 훨씬 간단하다고 들어서요. 구체적인 과정을 알고 싶은데 찾아봐도 번역이 어색해서 잘 모르겠네요ㅠㅠ 아래 글 바탕으로 알려주시면 감사하겠습니다!

1. (Note: you must work quickly here) Obtain six tubes of melted soft overlay agar from the water bath. Pipette 0.3 ml of a broth culture of E.coli into each of the soft agar tubes. Mix each tube well by rolling it between your palms. Label each tube with your initials and return them to the water bath as soon as possible. Do not allow the agar to solidify.
2. (Again work quickly) Remove one inoculated tube of soft agar from the water bath. Wipe off all the water from the surface of the tube. Using a 1 ml pipette, aseptically transfer 0.1 ml of the 10-1 saline phage dilution into the soft agar tube. Mix the agar tube by rolling it between your hands.
3. Immediately, aseptically pour the soft agar onto the surface of the nutrient agar plate correspondingly labeled as 10-1. Replace the lid and without picking up the plate, rotate it gently in a 6-to 8-inch circle on the surface of the table to evenly distribute the agar.
4. Using a fresh 1 ml pipette each time and working quickly, repeat steps 1 and 2 for the remaining saline phage dilution tubes and for the saline control tube.
5. For each dilution tube, use its correspondingly labeled nutrient agar plate.
6. Allow the soft agar to solidify.
7. Invert and incubate plates at 35°C to 37°C for 24 hours.